gal 9 rabbit mab  (Cell Signaling Technology Inc)

Journal: BMC Cancer

Article Title: The effect of Entamoeba histolytica lectin antigen and microRNA-643 on the development of microsatellite instability (MSI) in colorectal adenocarcinoma

doi: 10.1186/s12885-025-13472-x

Figure Lengend Snippet: Immunohistochemical staining pictures related to microsatellite instability are presented as follows: A depict positive staining for MutL homolog 1 (MLH1) and B&C depict positive staining for MutS Homolog 2 (MSH2) and MutS Homolog 6 (MSH6) mutations, while D show case negative staining for mutations of Postmeiotic segregation increased 2 (PMS2). Edge-enhanced and bright-field imaging ( E&F Imaging) of H&E staining. It is worth mentioning that in the analysis of immunohistochemical slides of microsatellite samples, the color ability of tumoral cell nuclei with chromogen indicates the absence of mutation in the investigated marker and is considered negative from the point of view of the specific antibody. In other words, the absovation of cells not stained with chromogen indicates a disruption in the DNA mismatch repair (MMR) system. MMR is a system for recognizing and repairing erroneous insertion, deletion, and mis-incorporation of bases that can arise during DNA replication and recombination, as well as repairing some forms of DNA damage G: Negative and H: Positive cytoplasmic staining image for Eh-lectin light chain antigen. I: H&E slide of adenocarcinoma of colorectal

Article Snippet: After cooling, the slides were incubated with an anti-Gal-lectin monoclonal antibody (HK-9 strain) from GeneTex to detect the specific Eh-lectin , followed by a blocking protein step.

Techniques: Immunohistochemical staining, Staining, Negative Staining, Imaging, Mutagenesis, Marker, Disruption

Journal: BMC Cancer

Article Title: The effect of Entamoeba histolytica lectin antigen and microRNA-643 on the development of microsatellite instability (MSI) in colorectal adenocarcinoma

doi: 10.1186/s12885-025-13472-x

Figure Lengend Snippet: The relationship between Adenocarcinoma, E. Histolytica - lectin and MSI

Article Snippet: After cooling, the slides were incubated with an anti-Gal-lectin monoclonal antibody (HK-9 strain) from GeneTex to detect the specific Eh-lectin , followed by a blocking protein step.

Techniques:

Journal: BMC Cancer

Article Title: The effect of Entamoeba histolytica lectin antigen and microRNA-643 on the development of microsatellite instability (MSI) in colorectal adenocarcinoma

doi: 10.1186/s12885-025-13472-x

Figure Lengend Snippet: Comparison of Eh-lectin antigen positive percentage in MSI positive and MSI negative group. The difference in Eh-lectin antigen positivity between the MSI + and MSI − groups was not statistically significant based on Fisher’s exact test ( P > 0.05). However, a significant difference was observed when comparing Eh-lectin antigen negative samples with positive and negative MSI status ( P < 0.009). Additionally, a significant difference was noted in the concurrent examination of MSI and Eh-lectin antigen ( P < 0.05). Out of the 39 MSI-positive samples, MSI + was observed in 3 samples, and there was a significant difference between these two groups ( P < 0.05)

Article Snippet: After cooling, the slides were incubated with an anti-Gal-lectin monoclonal antibody (HK-9 strain) from GeneTex to detect the specific Eh-lectin , followed by a blocking protein step.

Techniques: Comparison